Claudia Botti G.1 y Carlos MuñozS.2
Callus formation and its differentiation on vascular tissue in walnut (Juglans regia L.) stock cuttings (Eureka type) , scion (Eureka and Payne type) and non-rooted grafts, stratified in sawdust under controlled temperature conditions were studied. The main observations were:
- A great variation in callus formation, both in cuttings as in non-rooted grafts, was observed; however no anatomical differences were detected. This suggests that the physiological stage of the tissues is important when grafting.
- Callus formation was always greater in stocks than in scion, both in non grafted as in grafted cuttings. A less homogeneous and intensive callus evolution being observed in the latter.
- Tissues involved in callus formation were phellogen, cortex, phloem parenchymatic cells and vascular cambium, being the latter the major callus generator tissue.
- Differentiation on vascular tissue was irregular and started after 16 days of stratifieation, both on stocks cuttings as on grafted stocks.
1Ing. Agr., Profesor Adjunto Morfoanatomía, Facultad de Agronomía, Universidad de Chile, Casilla 1004, Santiago, Chile.
2Ing. Agr., Programa Frutales y Viñas, Estación Experimental La Platina, Instituto de Investigaciones Agropecuarias (INIA), Casilla 5427, Santiago, Chile.