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ANATOMICAL STUDY OF CALLUS FORMATION ON WALNUT (Juglans regia L.) CUTTINGS AND GRAFTS

Claudia Botti G.1 y Carlos MuñozS.2

Callus formation and its differentiation on vascular tissue in walnut (Juglans regia L.) stock cuttings (Eureka type) , scion (Eureka and Payne type) and non-rooted grafts, stratified in sawdust under controlled temperature conditions were studied. The main observations were:
- A great variation in callus formation, both in cuttings as in non-rooted grafts, was observed; however no anatomical differences were detected. This suggests that the physiological stage of the tissues is important when grafting.
- Callus formation was always greater in stocks than in scion, both in non grafted as in grafted cuttings. A less homogeneous and intensive callus evolution being observed in the latter.
- Tissues involved in callus formation were phellogen, cortex, phloem parenchymatic cells and vascular cambium, being the latter the major callus generator tissue.
- Differentiation on vascular tissue was irregular and started after 16 days of stratifieation, both on stocks cuttings as on grafted stocks.

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1Ing. Agr., Profesor Adjunto Morfoanatomía, Facultad de Agronomía, Universidad de Chile, Casilla 1004, Santiago, Chile.
2Ing. Agr., Programa Frutales y Viñas, Estación Experimental La Platina, Instituto de Investigaciones Agropecuarias (INIA), Casilla 5427, Santiago, Chile.

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