Milena G. Caccia1*, Eleodoro Del Valle2, Marcelo E. Doucet1, and Paola Lax1
Spodoptera frugiperda Smith and Helicoverpa gelotopoeon (Dyar) are important agricultural pests of several crops. The aim of the present work was to evaluate the susceptibility of larvae of both insectsto an isolate of Steinernema diaprepesi Nguyen & Duncan under laboratory conditions, as well as the capacity of the nematode to multiply on lepidoterans. Larvae (n = 15) were exposed to 0 (control), 50, and 100 infective juveniles (IJs) per Petri dish. Mortality was evaluated every 24 h during 6 d, and emerging IJs were counted. Mortality of S. frugiperda was 93% and 100% with 50 and 100 IJs dosage, and 87% and 93% in H. gelotopoeon, respectively. The production of IJs was significantly different between doses (P ≤ 0.05) for S. frugiperda (11329 with 50 IJs vs. 27155 with 100 IJs) but not for H. gelotopoeon (19830 vs. 26361, respectively). This is the first study evaluating the susceptibility of these lepidopterans to S. diaprepesi. These results encourage the possibility of using this nematode for biological control of both pests.
Key words: Infective juveniles, biological control.
1Universidad Nacional de Córdoba, Facultad de Ciencias Exactas, Físicas y Naturales, Rondeau 798, X5000, Córdoba, Argentina. *Corresponding author (email@example.com).
2Universidad Nacional del Litoral, Facultad de Ciencias Agrarias, 3080, Esperanza, Santa Fe, Argentina.